The Program has institutional infrastructure to conduct research, without external dependency. In addition to this structure, there is support from services such as:
- Rooms for theoretical classes and seminars: air-conditioned, equipped with microcomputers, multimedia projectors, DVDs, screens, tables and chairs.
- Rooms for qualifications and defenses: two air-conditioned rooms, with multimedia projectors, microcomputers, screens, upholstered chairs and a five-seater table for the examining board.
- Student lounge and teacher lounge: both air-conditioned, equipped with tables, chairs, microcomputers connected to high-speed internet (including wireless network), scanner and printer.
- Auditoriums: located in the Postgraduate building, they have capacities for 50, 150 and 600 seats, with complete infrastructure for courses and events.
- Postgraduate Office: provides support to students and professors on the program.
- Research Ethics Committees : Ethics Committee on the Use of Animals in Research (CEUA) and the Research Ethics Committee (CEP) are active and certified by the National Council for the Control of Animal Experimentation (CONCEA) and the National Council for Research Ethics (CONEP). They meet the demands of teachers and students regarding their projects involving human beings and/or animals.
- Library: The Indianópolis Library has specialized staff, accessible infrastructure, and areas for individual and group study. It has computers with internet access for research in national and international databases, as well as a diverse collection of books, periodicals, and multimedia. All UNIP libraries are interconnected online, allowing broad access to services and resources 24 hours a day. UNIP continually invests in expanding its collection, which currently has 1,720,314 items. In addition, the university provides access to online databases through the CAPES Portal and is part of the Federated Academic Community ( CAFe ), guaranteeing access to databases such as Web of Science, Scopus , Emerald , and Taylor and Francis.
In all these sectors, the Program has qualified employees trained for their specific functions.
Laboratories and clinics
The laboratory infrastructure is constantly improved based on the needs of researchers, through institutional support and assistance obtained from funding agencies, industries and the third sector.
Since 2010, when the Program was restructured, the University has invested more than R$3 million in an updated research center and clinic that are adapted to the needs of research carried out in various clinical and laboratory areas. This infrastructure was added to the existing research laboratories at UNIP, which are shared with the Graduate Program in Environmental and Experimental Pathology.
In order to improve the infrastructure for laboratories, in 2020 the University invested in the construction of a new research center, measuring 962.56 m2 , which was inaugurated in 2021. Below, we present the infrastructure made available to the Program.
1. Outpatient Care Center and Digital Technology Laboratory (3D) : built next to the dental clinic building in 2019, after the formal establishment of a partnership with Instituto Mais Identidade (+ID), an NGO (OSCIP) dedicated to serving patients with maxillofacial deformities resulting from cancer treatment or traumatic sequelae. The partnership established that the construction of the physical space would be carried out by UNIP and that Instituto +ID would bring equipment acquired through donations from private companies (Rede IMPAR and Banco Itaú). The complex has been in operation since October 2020 and has a scanner intraoral and benchtop machines, three 3D printers, complete equipment for a conventional prosthetic laboratory (microwave ovens, refrigerators, implant motors, electric motors, instruments and materials for prosthetics, among others), as well as complete dentistry equipment, such as dental chairs and equipment. The Institute also has a special room, called a “color room ”. This room has a lighting system that is unprecedented in Brazil, which allows the capture of patients’ skin color with minimal distortion, providing aesthetic results that are far superior to conventional standards. The space is used by professors and students who work in the care and rehabilitation of the population affected by head and neck cancer, as well as patients who need prosthetic rehabilitation, linked to the research projects developed by the Program. This partnership subsidized important products, mentioned below, which strengthen the social impact of the Program.
2. Dental Research Laboratory : the laboratory has equipment purchased by the Institution, as well as through assistance obtained from funding agencies. This equipment allows for the execution of research projects in the various subareas of knowledge that make up the area of concentration in Clinical Dentistry, in addition to analyses in molecular biology, which support clinical and animal research. The equipment is listed below:
1. JEOL scanning electron microscope JSM-6510; 2. JEOL Desk V Metallizer ; 3. JEOL Desk Yarn Carbon Vaporizer (R$ 287,419.24 – UNIP 2011 grant); 4. Future-Tech FM-700 Microhardness Tester (R$ 140,000 – FAPESP 2010); 5. KRATOS KE 2.000MP Universal Testing Machine (R$ 38,400 – UNIP 2011); 6. Luminex MAGPIX Millipore (R$ 112,500 – FAPESP 2011); 7. Buehler IsoMet 1000 Cutter (R$ 24,508 – UNIP 2010); 8. Fume Hood (R$ 1,900 – UNIP 2010 grant); 9. Thermoplate Reader Tp-reader (R$ 19,500 – FAPESP 2011); 10. Extensometer (R$ 35,000 – FAPESP 2012); 11. Gehaka AG200 analytical balance; 12. Centribio 80-2B centrifuge; 13. Labtech water bath for 60 tubes; 14. Permution fume hood ; 15. Gehaka BK3000 electronic balance ; 16. Biomixer 78HW-1 magnetic stirrer ; 17. Vortex stirrer Biomixer QL-901; 18. Ultrasonic bath; 19. Maxipress sample filler Solotest ; 20. Maxicut electric cutter Solotest ; 21. Polisher Maximille Solotest ; 22. Fanem drying and bacteriological ovens (81 and 150 L); 23. Digital caliper; 24. Deionizer ; 25. Peaggmeter ; 26. Semi-automatic pipettors ; 27. ELISA reader; 28. Roughness meter Surtonic 25; 29. Intra-Spin Centrifuge INTRA-LOCK (R$ 4,427.02 – FAPESP 2019); 30. Minicentrifuge K14-060 Kasvi (R$ 1,099 – FAPESP 2016/12758-3); 31. Water bath 201010 Thoth Equipamentos (R$ 1,500 – Asset 01192230); 32. Nanodrop lite – Spectrophotometer – Thermo Fisher Scientific (R$ 17 thousand – FAPESP 2013); 33. Minicentrifuge MCA (R$ 1,100 – FAPESP 2013); 34. Digital water bath (R$ 1,600 – FAPESP 2013); 35. Freezer (R$ 1,600 – FAPESP 2014); 36. Stereomicroscope C-os – Nikon Instruments Inc. (R$ 7 thousand – FAPESP 2014); 37. Precision balance (R$ 2 thousand – FAPESP 2014); 38. Electrophoresis tank (R$ 1,465 – FAPESP 2014); 39. Minicentrifuge (R$ 840 – FAPESP 2014); 40. Vortex (2 x R$ 637 – FAPESP 2014); 41. Microwave (R$ 560 – FAPESP 2014); 42. MINI-V Vertical Laminar Flow Cabinet – Telstar (R$ 13 thousand – CNPq 2015); 43. Real-Time PCR System – LightCycler 96 – Roche (R$ 90 thousand – FAPESP 2015); 44. Magnetic stirrer with heating (R$ 1,700 – FAPESP 2014); 45. Photopolymerizer Bluephase G2 Ivoclar (R$ 5 thousand – FAPESP 2014/17878-1); 46. Dell i7 Notebook (R$ 5,200 – FAPESP 2014/17878-1); 47. Micro-homogenizer (R$ 2,850 – FAPESP 2016); 48. Mini plate spinner (R$ 3,350 – FAPESP 2016); 49. MCA 1-14K Centrifuge – Sigma (2 units) (R$ 63,500 – FAPESP 2016); 50. Micropipettes (R$ 12,690 – FAPESP 2013/2014); 51. 6400RPM Bivolt Minicentrifuge (R$ 1,099.00 – FAPESP 2016/12758-3); 52. ChiroPro implant motor – Gnatus ®️ (R$ 10,350 – RT Institutional Grant Process 2015/13894-5).
3. Cellular and Molecular Biology Laboratory : divided into three subareas: (1) cellular biology, (2) molecular biology, and (3) oral and infectious agents. These laboratories work in harmony to develop research in the various clinical and basic areas of the Program. Examples include the study of cellular and tissue physiology and biochemistry ( in vitro , animal studies, and clinical studies) to understand molecular mechanisms and their subsequent application in the medical and biotechnological areas. The study of these biological models, with or without infectious agents, provides answers to questions about biomaterials , toxic and infectious agents, antioxidants, drugs, and pathological and physiological conditions, for subsequent characterization in specific equipment.
In addition to consumables, benches and other basic facilities, the laboratory has the following equipment:
Acquired in the 2021–2024 four-year period :
1. FTIR spectrophotometer model iS50 – Thermo Fisher Scientific (USA) (US$ 70,630.51/R$ 349,416.20 – FAPESP EMU Grant – Multiuser Equipment 2023/05652-8; 2. Programmable sonicator , FISH-FB120110 Model 120 – Fisher Scientific (USA) (US$ 3,150 – FAPESP 2022/04770-4); 3. Rotary mixer for tubes, BENCH-r5010 1.5/2.0/15/50 ml – Benchmark Scientific (USA) (US$ 1,116.02 – FAPESP 2022/04770-4); 4. OPTI-CB5 – B5 camera, 5.1 MP, Optika Microscopes (USA) (US$ 368.09 – FAPESP 2022/04770-4); 5. OPTI-M620 – Adapter with microscope mount (US$ 180.91 – FAPESP 2022/04770-4); 6. Ultrafreezer -86 °C INDREL – Model IULT 335D (R$ 31,800 – FAPESP 2022/04770-4); 7. Genomic sequencer NANO-MINION BASIC PACK – Nanopore Technologies (USA) (US$ 1,250 – FAPESP 2022/04770-4).
Acquired until 2021 :
1. MAGpix ( Luminex Corporation); 2. Tube shaker, autoclaves (Phoenix); 3. Analytical balance ( Gehaka ); 4. Class II A1 cabin for handling microorganisms ( Pachane ); 5. Digital camera for gel “ photodoc ” (SP 500-UZ – Olympus); 6. Excelsa Baby I centrifuge (Fanem); 7. Refrigerated centrifuge (5804R – Eppendorf ); 8. TE2755 distiller ( Tecnal ); 9. Three bacteriological incubators (two 502 – Fanem and one AL510 – American Lab ); 10. Two sterilization incubators (AL500 – American Lab ); 11. Two electrophoresis sources (EPS301 – Amersham Biosciences ); 12. Four upright freezers (240 – Brastemp); 13. Five refrigerators (411L – DF48 – Electrolux); 14. Fume hood with LCD display for gel documentation (Feldman Wild Leitz ); 15. CO 2 incubator (TE399 – Tecnal ); 16. Ice machine (EGE160 – Everest); 17. Three orbital shaking tables (TE141 – Tecnal ); 18. Microcentrifuge ( MiniSpin Plus 5453 – Eppendorf ); 19. Trinocular microscope ( PrimoStar – Zeiss ); 20. Phase contrast and fluorescence microscope ( Axiovert 40C – Zeiss ); 21. Two horizontal mini-cuats for electrophoresis (HE33 – Amersham Biosciences ); 22. Microwave (NHS56BH – Panasonic); 23. Micropipettes (FAPESP 06/61171-3), five of 0.5–10 µL ( Finnipipette – Labsystems , epResearch 10 – Eppendorf , Microman M10 – Gilson), four of 10–100 µL ( epResearch 100 – Eppendorf and epResearch 2100 – Eppendorf ), four of 100–1,000 µL ( epResearch 1000 – Eppendorf and epResearch 2100 – Eppendorf ), two of 2–20 µL ( Pipetman P200 – Gilson), two of 30–200 µL ( Pipetman P200 – Gilson), two of 250 µL ( Pipetman F250 – Gilson), two of 1,000 µL ( Pipetman F1000 – Gilson), one of 10–50 µL (3002 – Oxford), one of 200–1,000 µL (3004 – Oxford); 24. Multichannel micropipette (eight channels 5–500 µL – Boeco ); 25. Photo/UV transilluminator 21 (3-3025 – Fotodyne ); 26. Reverse osmosis and Milli -Q ( Direct Q3 – Millipore); 27. Microprocessor-based benchtop digital pH meter (PG1800 – Gehaka ); 28. Pipet-Aid ( Midi Plus BioHit – FAPESP 06/54343-2); 29. Fixed-angle rotor (30 tubes of 1.5–2 mL and six tubes of 85 mL F45-30-11 – Eppendorf ); 30. Gel analysis system ( GelQuant v2.7 – Bio-Imaging Systems); 31. Two thermocyclers ( Mastercycler and Mastercycler Gradient – Eppendorf ).
4. Bioterium and Experimentation and Animal Behavior Laboratories : the bioterium is intended for experiments with rats, mice, fish and aquatic invertebrates. With an initial investment of approximately US$ 200 thousand, it was structured with rooms for carrying out experiments, behavioral analysis, surgery, perfusion, pathology, cell culture, cytometry ( Accuri ), study of infectious diseases, image analysis and support rooms for teachers, students and technicians. The bioterium at UNIP is the SPF ( Specific) model. Pathogen Free ), as internationally established in terms of quality. To meet these specifications, UNIP invested approximately US$ 300 thousand in equipment and infrastructure. The technical and administrative infrastructure of the vivarium is prepared to receive and maintain the animals during the experimental period and is in accordance with the guidelines established by CONCEA, which allows compliance with all requirements for research with experimental animals, with a view to their well-being. The vivarium has equipment such as laminar flow cabinets specific for protocols with genetic material, thermocyclers , CO2 incubators , refrigerated incubators, thermoblocks , thermostated baths , pH meter, refrigerated centrifuge, ice machine, ELISA reader, specific flows for research with fungi and bacteria, incubators, BOD, refrigerators and freezers, microscopes, centrifuges, scales, among other equipment necessary for a level 2 safety laboratory. More recently, instruments measuring murine vocalization , microisolators ( Techniplast ) specific for keeping rats and mice in environments with controlled temperature and humidity, equipment for behavioral analysis, such as different arenas for motor, anxiety, depression tests, among others; video cameras, monitors and Noldus-Observer software , photomicroscope (Nikon) and Metamorph image analyzer (Molecular Devices ) were incorporated into the equipment park.
5. Biodiversity Research Center ( NPBio ) of UNIP : in 2007, the Program established a partnership with NPBio in order to diversify research activities. Projects are developed with extracts of Brazilian plants, mainly from the Amazon Rainforest and the Atlantic Forest, and, more recently, with plant species from Antarctica. This Center includes the Extraction, Antitumor Screening, Cell Culture, Microbiology, Chemistry of Natural Products Laboratories and the UNIP Herbarium, all located in São Paulo, and the Plant Processing Laboratory, located on the Manaus campus. The Biodiversity Research Center uses a boat owned by UNIP to conduct field expeditions. The UNIP Herbarium is registered with the Index Herbariorum , an important international entity that regulates activities in the area. The equipment of the Antitumor Screening Laboratory, as well as the consumable material used in the experiments, were financed by FAPESP (US$ 104,525.97). It is worth noting that all collection of botanical material carried out by researchers at the Extraction Laboratory is carried out with a license issued by the Brazilian Institute of the Environment and Renewable Natural Resources (IBAMA, Ministry of the Environment) and by the Genetic Heritage Management Council ( CGen ), and that all biological or chemical tests with extracts obtained from plants collected in conservation units are performed under a license for access to genetic resources (MP No. 2,186, of September 2002), which legitimizes the research work carried out by the Institution. Although access to genetic resources and all collections carried out by UNIP are done with the aforementioned authorizations, today all collection material is being entered into SisGen , the national registry of access to genetic resources, in accordance with new government guidelines (Decree No. 8,772, of May 11, 2016).
5.1. Extraction Laboratory : designed to obtain plant extracts from plants in the Amazon and Atlantic Forest regions, which will be evaluated for their therapeutic properties, based on in vitro and in vivo studies . The laboratory has the following equipment: Walk -In hoods; knife mill (Thomas); an oven with air circulation (Fanem); two analytical balances ( Ohaus and Mettler ); an electronic balance (Marte); a Milli -Q water purification system (Millipore); five rotary evaporators ( Buchi ); a vacuum pump (Edwards); an ultrasonic bath; a manifold for drying samples; a chiller coupled to the rotary evaporators ( Neslab ); a -20 °C cold chamber; a -70 °C freezer ( Revco ); and two tray freeze dryers ( Virtis and Labconco ).
5.2. Microbiology Laboratory : intended for analyses related to the identification of plant extracts active against bacteria of importance to humans and animals, particularly those used in dentistry. The laboratory has two temperature-controlled incubators (Fanem and De Leo); a laminar flow chamber ( Veco ); an optical microscope ( Zeiss ); a micro-library of microorganisms: Staphylococcus aureus, Streptococcus mutans , Streptococcus sanguinis, Enterococcus faecalis , Candida albicans , Escherichia coli, Pseudomonas aeruginosa , Porphyromonas gingivalis and Prevotella intermedia; six multichannel variable volume micropipettes ( Mettler and Eppendorf ); one automatic pipettor ( Eppendorf ); two domestic refrigerators (Electrolux); one microwave (GE); three desiccators; anaerobic systems; one turbidimeter ; pH meter ; and consumables and glassware.
Phytochemistry Laboratory : intended for fractionation and purification of substances from plant extracts. The laboratory has: a plate preparer; a UV-VIS spectrophotometer ( Femto ) coupled to a computer; glass chromatographic columns of various sizes; specific reagents; two hoods; silica for chromatoplates and columns; Sephadex LH20; silica C-18 for columns; HPLC syringes ( Phenomex ); glassware; solvents; reagents; three magnetic stirrers with heating; UV development lamp 254 and 366 nm ; hair dryer; capillaries; a high-performance analytical liquid chromatograph ( Agilent ), and a gas chromatograph ( Agilent ).
5.4. Antitumor Screening Laboratory : intended for cytotoxicity assays to identify extracts that inhibit the growth of human tumors, in vitro , according to international standards. The laboratory equipment, as well as the consumables used in the experiments, were financed by FAPESP (amount: US$ 104,525.97). The laboratory has a centrifuge ( Eppendorf ); a refrigerator (Electrolux); a freezer (Electrolux); a 96-well microplate reader ( Biotek ); a 96-well microplate washer ( Biotek ); an automatic pipettor ( Eppendorf ); two multichannel micropipettes ( Biohit ) and Eppendorf pipettes ; a liquid nitrogen cylinder; an optical microscope ( Zeiss ); an inverted microscope ( Zeiss ); a double incubator (Forma); a BL-2 laminar flow chamber (Forma); a pH meter ; a microplate shaker and consumables.
6. Postgraduate Dental Clinic : intended for clinical research and patient care. The clinic, which is air-conditioned, has 20 fully equipped dental offices, as well as implant motors, a clinical microscope, a CT scanner, a digital X-ray machine and all the basic infrastructure for its operation.
7. Oral Cancer Detection and Prevention Center (CDPCP) : has a fully equipped dental office, suitable for surgical procedures for diagnosis and treatment, as well as equipment for filming and recording interventions, with the possibility of live transmission to the theater, amphitheater and/or undergraduate and postgraduate classrooms. The CDPCP was developed through a partnership with the “Head and Neck Surgery” Discipline of the Paulista School of Medicine (EPM – Unifesp) and allows diagnosed cases to be referred directly to this specialized oncology service. This facilitates access to care, reduces waiting times and gives patients much better chances of cure and quality of life. At the CDPCP, undergraduate and postgraduate students work under the guidance and supervision of professors from the Program, developing not only assistance activities, with great social impact, but also scientific initiation and postgraduate research.
8. Dental Laser Center : contains a high-power Nd :YAG laser (FAPESP 95/05322-6) and four low-power devices ( GaAl As Diode ; Diode associated with LED), which are used in clinical research, in vitro research and patient care.
9. Surgical Center : has rooms equipped for procedures with intravenous or nitrous oxide sedation, changing rooms for patients and professionals, purging and antisepsis rooms, as well as equipment for filming and recording interventions, with the possibility of live transmission to the theater, amphitheater and/or postgraduate and undergraduate classrooms.
10. Sterilization Center with autoclaves and ovens .
11. CEAPE – Center for Studies and Care for Special Patients : has dental equipment and is a reference for referring patients seeking the STD-AIDS Program of the City of São Paulo. The Center also cares for patients with other special needs and conducts clinical research.
12. Laboratories of the More Identity Institute (unip.br/cursos/pos_graduacao/strictosensu/laboratorios/mais_identidade.aspx)
12.1. Digital Laboratory for Prosthesis Production: This laboratory has 3D printers, scanners and state-of-the-art computers, allowing the creation of facial and intraoral prostheses with high precision. Using models obtained through digital photographs, it is possible to sculpt personalized prostheses, facilitating care for patients in different locations, including remotely.
The prostheses are digitally modeled from scanned images , ensuring a perfect fit and greater comfort for the patient.
The technology used enables a more agile and personalized process, in addition to reducing costs and optimizing resources.
12.2. Conventional Prosthesis Laboratory: Although digital technology has revolutionized the production of prostheses, the conventional laboratory is still essential for the refinement and completion of the pieces.
Here, professionals perform manual adjustments and personalized finishes, ensuring the aesthetics and functionality of the prostheses.
This team works in an integrated manner to offer complete rehabilitation, promoting aesthetic and functional recovery, in addition to social reintegration.
They are also responsible for guiding patients on how to care for their prosthetics and adapt to their new look.
12.3. Photography and Scanning Laboratory (Color- Room ): This space is one of the most innovative in the Institute, being essential for the planning and execution of facial prosthetics using digital technology.
The room is equipped with advanced image capture systems, ensuring that every anatomical detail is faithfully reproduced on the prosthesis.
Digital scanning allows for detailed planning, enabling the production of prosthetics with an extremely natural appearance and personalized for each patient.
12.4. Teaching and Training Laboratory: Focused on the education and training of professionals in rehabilitation techniques, this laboratory also promotes scientific exchanges and workshops, contributing to the continuous updating and improvement of professionals in the field.
12.5. Care Clinics : Care clinics are equipped for patient consultations and assessments, allowing detailed monitoring throughout treatment.
They have a multidisciplinary team made up of dentists, doctors, speech therapists, psychologists, nurses, physiotherapists, social workers and nutritionists.
In addition to clinical support, these rooms provide guidance to patients on how to care for their prostheses and adapt to their new look.
12.6. Surgical Center : The Institute has a surgical center prepared to perform oral and maxillofacial rehabilitation procedures.
It is in this environment that facial reconstruction surgeries and the installation of bone-integrated implants take place.
The modern structure allows for safe and effective procedures to be carried out, which are essential for the functional and aesthetic recovery of patients.
These laboratories and clinical spaces are essential for Instituto Mais Identidade to achieve its mission of offering high-quality aesthetic-functional rehabilitation, promoting the recovery of self-esteem and the social reintegration of patients.
Multi-User Equipment
Equipment: Fourier Transform Infra-Red Spectrometer (FTIR)
Brand: ThermoFischer
Model: IS50
Origin: Foundation for Research Support of the State of São Paulo (FAPESP)
Responsible Researcher: Adriano Fonseca de Lima
Description
Fourrier Transformed Infra-Red Spectrometer is a device used for the characterization of materials and analysis of
chemical reactions, both of solid and liquid materials, by obtaining mid- and near-infrared spectra.
Equipment Location
Research Laboratory of the Paulista University - Rua Dr. Bacelar 902.
Equipment Use
To use the device, it is necessary to make an appointment in advance via email: pgodonto@unip .br. It is important to emphasize that the scheduling is subject to the availability of the team responsible for the equipment.