Apresentação

Programa de Pós-Graduação Stricto Sensu em Patologia Ambiental e Experimental

  • Promote the training of researchers with national quality standard scientifically to the needs of the field of Environmental and Experimental Pathology.
  • Training of researchers/professors who meet the high demands of society with scientific vision and analytical thinking, according to recognized academic and ethical principles.
  • Qualify teachers for higher education, grade school, and basic school.
  • Assign the title of Master and / or Doctor in Environmental and Experimental Pathology.

Upon completion of the program, it is expected that the student Egress:

  • Be able to formulate research questions, plan and conduct High international caliber research and academic development work within your field.
  • Learn how to use scientific equipment, such as instruments and analysis tools in your area of expertise.
  • Get the publication of the article(s) published in national and international magazines and newspapers.
  • Be able to deal with complexity, create an overview, and synthesize scientific data.
  • Be able to carry out critical and constructive analyzes of scientific work in your field.

Veterinary surgeons, biomedical physicians, nurses, physicians, pharmacists, physiotherapist, biochemists, biologists, nutritionists and similar areas.

The Environmental and Experimental Pathology Program (PAE) meets the growing demand for knowledge in the field of health environmental, evaluating the impact that environmental bring aabout on the origin of diseases and health problems, with this area having had little exploration in Veterinary Medicine.

The concept of environmental health considers all the elements that potentially affect health and studies the diseases caused by pathogens introduced into the environment by the action of and man associated with more subtle adverse factors such as the social, economic, political and institutional character. Thus, environmental changes, such as water crises, urbanization and growth of cities, deforestation, invasion of green areas, presence of chemicals in the environment, climate changes, among others, affect the host-parasite relationship and favor the occurrence of diseases.

The research lines of the program cover two areas still poorly explored in the Post-Graduate Programs in the area of Veterinary Medicine throughout Brazil: Environmental Pathology and Experimental Pathology, which meet the need for training human resources for the complex area of sciences applied to the field and health, which Veterinary Medicine is a part of. With a multidisciplinary profile, the faculty includes professionals in the areas of: Veterinary Medicine, Biology, Biomedicine, and Pharmacy.

The Post-Graduate Program’s ORGANIZATIONAL IDENTITY in PAE is sedimented into the following aspects:

  • MISSION: train professors and researchers from the areas of health, agrarian sciences and the environment, allowing multidisciplinary training and the generation of knowledge in a critical, current, innovative and humanistic way, in the area of Environmental and Experimental Pathology, in line with the constant demands of society.
  • VISION: consolidate the PAE program and the participation of the Paulista University in the scope of national and international research by 2025.
  • ORGANIZATIONAL VALUES: ethics, inclusion, innovation and creativity, sustainability, environmental responsibility, transparency, self-criticism, solidarity and national and international cooperation.
    The faculty of the Program (Collegiate) exercises a shared/participatory management with the elaboration of the STRATEGIC PLANNING of our Program, focusing on national and global issues that is imposed in continuous flow. It is worth highlighting the realization of SELF-ASSESSMENTS has directed decision-making and self-management of the Program, providing elements for the discussion of educational and structural propositions that consolidate the University as a center of excellence in the training of professionals.

Agricultural Science

Knowledge Area

Environmental and Experimental Pathology

Concentration Area

Master's and PhD in Environmental and Experimental Pathology

1. Integrated and Translational Pathology (Professors: Anete, Borelli, Eduardo, Elizabeth, Guilherme, Ivana, Mario, Martha, Paulo, Selene, Thiago).
In this Line of Research, experimental and clinical studies are developed for the understanding of the physiopathogeny and treatment of diseases and disorders related to environmental factors, such as neurodegenerative diseases caused by stress, toxic, pharmacological, nutritional or biological agents, neglected and reemerging infectious diseases or related to variations in immunocompetence, neoplastic diseases and mental disorders. The One World-One Medicine-One Health approach, involving environmental, human and animal factors, is the basis of the research contemplated in this Line.

2. Ecotoxicology and Therapeutic Innovations (Professors: Eduardo, Elizabeth, Giovani, Ivana, Leoni, Martha, Paulo, Thiago).
In this line of research, the studies seek to understand the evolution of diseases and disorders in different animal species that may result from anthropic interferences in nature, from exposure to toxic agents. At the same time, it aims to find sustainable therapeutic solutions for these and other problems of human and veterinary interest, from plant extracts (especially those from the Atlantic Forest and the Amazon, Brazilian biomes with a high degree of biodiversity) and new uses for existing drugs, in addition to elucidating the mechanisms of action of streamlined inputs (or highly diluted homeopathic products), which are used in Brazilian agriculture. Substances that interfere with the neuro-immune-endocrine axis and the use of experimental and behavioral models are also objects of study of this line.

According to the Lines of Research and in view of the program proposal, the specific objectives are:
1. Evaluate environmental factors and their relationship with the occurrence of chronic and acquired diseases with the use of experimental models (e.g., breast adenocarcinoma, melanoma, Ehrlich tumor, streptozotocin-induced diabetes, chemotherapy-induced cognitive dysfunction, among others) and by the analysis of epigenetic factors and their correlation with the onset of diseases.
2. Analyze the risk factors present in the environment, such as contamination by chemicals, pesticides, sunscreens, bacterial substrates, and their relationship with psychiatric and behavioral disorders, using classical models of behavioral assessment in laboratory rodents and by introducing innovative models in laboratory animals such as fish and microcrustaceans, such as zebrafish (Danio rerio) and Artemia salina.
3. Study the natural history of bacterial, fungal, parasitic, and viral infectious diseases in our country, covering urban, rural and wild areas, by evaluating data on the prevalence and incidence of pathogens and their genotypic variants in the most diverse species of Brazilian fauna, by the pathogenesis of infectious agents in conventional and innovative experimental models, by the development of new forms of cell culture to maintain infectious agents , by the use of new diagnostic methods.
4. To investigate the existing therapeutic activity in new compounds extracted from Plants of the Amazon and the Atlantic Forest in experimental models in vivo and in vitro, particularly through tests in cell cultures, microorganisms, tumor cells, animal models and biochemical methods.
5. Evaluate dynamic insumable and high dilutions (homeopathic compounds) regarding their mechanisms of action and as therapy against infectious, parasitic and neoplastic diseases, as well as evaluation of their zootechnical, phytotechnical or eco-remediation potential, in promoting the rebalancing of relations between living beings and the environment, both subject to anthropic disorders.
6. Analyze the different aspects of the immune response, with particular emphasis on the activity of B-1 cells and their communication with other cells of the immune system, innate and acquired immunity in microsporidia infections and activity against experimental tumors.
7. Study morphological, biochemical, behavioral and epigenetic changes in diseases of the central and peripheral nervous system.

  • Specialized library, structured to support teaching and research, featuring sectoral libraries with a central information unit, connecting all campuses, via intranet.
  • Veterinary Hospital, located on Campus Anchieta and which provides an important subsidy for the development of the Program's research, enabling research not only with companion animals, as well as medium and large animals.
  • Pathological Anatomy Laboratory, located at the Research and Technology Center, with adequate infrastructure for histological processing of material from biopsy or necropsy, both for diagnostic and of research purposes. Histochemical and immunohistochemical techniques are also available.
  • Laboratory for Extraction of Active Principles from Brazilian Plants
  • Laboratory for experimentation in homeopathy: In the facilities of the Research and Technology Center, basic and applied research on ultra dilutions and homeopathy is carried out. From raw material acquired from sources accredited by ANVISA, different homeopathic potencies can be produced in the laboratory in an automated way, for immediate use in animals or cell cultures.
  • Laboratory of Molecular and Cellular Biology: It was built with the purpose of supporting different research. It is divided into 3 sub-areas: Cell Biology, Molecular Biology and Infectious Agent Research. In these laboratories they have equipment compatible with the application of molecular biology techniques for diagnosis and research, development of microorganisms for research, bacteriological and mycological cultivation, among others.
  • Cell Culture Laboratory: The structure allows the maintenance of permanent cell lines such as RK, Vero, MDCK cells, macrophages, melanoma tumor cells and mammary adenocarcinoma, creating a structure for the development of several projects in vitro.
  • Animal Experimentation Laboratory (SPF) and Fish Vivarium: The vivarium follows international requirements for maintaining rats and mice for experimentation under SPF (specific pathogen free) conditions, where several models and protocols for behavioral, toxicity studies have been standardized, infectious diseases and experimental tumors. Recently, the Fish Vivarium was implemented, meeting a new demand for biological models to replace conventional animals and which will make it possible to expand research using the pattern zebrafish model.
  • Cryopreservation Laboratory, containing ultra low freezer, liquid nitrogen tanks and a fluorescence microscopy room.
  • Flow Cytometry Laboratory, recently, a flow cytometer was acquired in order to improve cellular techniques for studies in immunology and pathology.

a) Institutional Partnerships and Covenants

Professor: Eduardo Fernandes Bondan
Partnership: Prof. Ph.D. Rosemari Ottoni - Laboratory of Natural Antioxidants - University Cruzeiro do Sul (São Paulo - SP)
Project involved: Study of morphological, oxidative and behavioral effects of doxorubicin administration associated with curcumin and resveratrol in Wistar rats

Partnership: Transmission Electron Microscopy Laboratory, Department of Anatomy, College of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ – USP)
Project involved: Morphological study of the demyelinating and remyelinating process in the brainstem of XID mice submitted to the gliotoxic model of ethidium bromide

Partnership: Dr. Marcelo Paes de Barros (Institute of Physical Activity and Sport Sciences - ICAFE / University Cruzeiro do Sul)
Project involved: Study of oxidative parameters in the central nervous system of rats treated with doxorubicin

Partnership: Dr. Miriam Oliveira Ribeiro (Neurobiology Laboratory / Presbyterian University Mackenzie)
Project involved: Induction of iodothyronine deiodinase after status epilepticus modifies hippocampal gene expression in male mice

Professor: Elizabeth Cristina Pérez Hurtado
Partnership: Prof. Ph.D. Rosemari Ottoni - Laboratory of Natural Antioxidants - University Cruzeiro do Sul (São Paulo - SP)
Project involved: Study of morphological, oxidative and behavioral effects of doxorubicin administration associated with curcumin and resveratrol in Wistar rats

Partnership: Transmission Electron Microscopy Laboratory, Department of Anatomy, College of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ – USP)
Project involved: Morphological study of the demyelinating and remyelinating process in the brainstem of XID mice submitted to the gliotoxic model of ethidium bromide

Partnership: Dr. Marcelo Paes de Barros (Institute of Physical Activity and Sport Sciences - ICAFE / University Cruzeiro do Sul)
Project involved: Study of oxidative parameters in the central nervous system of rats treated with doxorubicin

Partnership: Dr. Miriam Oliveira Ribeiro (Neurobiology Laboratory / Presbyterian University Mackenzie)
Project involved: Induction of iodothyronine deiodinase after status epilepticus modifies hippocampal gene expression in male mice

Professor: Ivana Barbosa Suffredini
Partnership: Prof. Ph.D. Cristina de O. Massoco S. Gomes – Department of Pathology – FMVZ-USP
Project involved: Microenvironment and tumor aggressiveness: cancer-associated fibroblasts (CAFs), pericytes and prognosis in canine oral melanoma

Partnership: Prof. Ph.D. Isaltino Marcelo Conceição – Butantã Institute
Project involved: Evaluation of tumor growth and its microenvironment in mice submitted to schizophrenia model and breast carcinoma patients

Professor: Leoni V. Bonamin
Partnership: Prof. Elizabeth Teodorov - Federal University of ABC
Project involved: Effects of isotherapeutic drugs on experimental intoxication of Artemia salina

Partnership: Prof. Carla Holandino - Federal University of Rio de Janeiro
Project involved: Physicochemical characterization of homeopathic medicines

Partnership: Prof. Silvia Waisse - Pontifical Catholic University of São Paulo
Project involved: International book publishing

Water project to be presented at the next World Water Forum in Dakar, in March 2022. See details here.

Professor: Maria Anete Lallo
Partnership: Butantan Institute, Pathophysiology Laboratory, for transmission electronic microscopy, Dr. Diva Denelle Spadacci-Morena
Project involved: Morphodifferentiation of the Gené organ of engorged ticks Amblyomma sculptum (Acari: Ixodidae)

Partnership: Immunology Laboratory, Faculty of Pharmaceutical Sciences, State University of Araraquara, Prof. PhD. Alexandra de Medeiros
Project involved: Murine microsporidiosis – the role of M1 and M2 macrophages, Phagocytes derived from B-1 cells and efferocytosis

Partnership: Cellular Biology and Immunology Subjects of the Department of Microbiology and Immunology – Federal University of São Paulo, Prof. Ph.D. Alexandre Keller
Project involved: Role of NKT cells in encephalitoszoonosis

Partnership: Department of Micro-, Immuno-Parasitology Federal University of São Paulo and Institute of Environmental, Chemical and Pharmaceutical Sciences (ICAQF) Federal University of São Paulo Diadema Campus, Prof. Ph.D. Patrícia Xander Batista.
Project involved: Influence of B-1 cells or their extracellular vesicles on the activity of effector immune cells in microsporidiosis

Professor: Maria Martha Bernardi
Partnership: Dr. Claudia Madalena Mori - Department of Pathology- FMVZ / University of São Paulo
Project involved: Phenotypic characterization of spontaneous mutant mice induced by ENU

Partnership: Sandra Maria Miraglia Valdeolivas - Paulista School of Medicine – EPM/UNIFESP Department: Morphology and Genetics
Project involved: Effects of moderate chronic stress, induced from peripuberty, and treatment with escitalopram on reproductive parameters of adult rats.

Professor: Paulo Ricardo Dell’Armelina Rocha
Partnership: Department of Pathology - FMVZ- USP
Project involved: Laboratory techniques and tests, examination of pathological report, molecular and morphological aspects

Partnership: Department of Clinic, Surgery and Animal Reproduction - UNESP Araçatuba. Post-Graduate Program in Animal Science
Project involved: Laboratory techniques and tests, examination of pathological report, molecular and morphological aspects

b) International exchanges
Profa. Maria Anete received student Alicia Herrera, from the Masters in Veterinary Medicine at the Universidad Autónoma de Nuevo León in Mexico, under the supervision of Prof. Dr. Uziel Castillo Velázquez. His stay in Brazil enabled the international cooperation agreement between Universidade Paulista and Universidad Autónoma de Nuevo León, which will be consolidated with the completion of the Doctoral project with and the arrival of Alicia Herrera in 2020, involving the participation of Dr. Velázquez, as associate professor. In Mexico, Professor Dr. Velázquez's group is the only one working with microsporids in the area of ​​Veterinary Medicine, especially in the breeding of rabbits. In Brazil, our group is a reference in microsporidia, so this agreement will benefit the internationalization and strengthening of the 2 groups. There are already negotiations for the professors involved to travel between Universities to participate in boards, idealization of projects, presentation of lectures. Thus, we hope that the realization of this project and the internationalization agreement will strengthen the 2 existing research groups in the Americas, excluding the well-consolidated groups in the United States, enabling the idealization of a research network with microsporidioses and expanding the visibility of Brazil. Thus, the Program demonstrates its capacity to receive foreign students for postgraduate and training courses, reinforcing its scientific maturity.
International collaboration is of vital importance for the scientific advancement of any nation. The Scholarship Abroad Program, developed by CGBE, in the “sandwich” mode, allowed the consolidation of an international link with Università di Verona, as described below. Additionally, teachers / researchers develop other collaborations that reflect the internationalization of the Program.

Professor: Leoni V. Bonamin
- General Pathology - Università di Verona - Italy.
- Institution: Global Homeopathy Foundation (GHF) - Mumbai - India.
- Royal London Hospital for Integrated Medicine (RLHIM) - London - UK.
- Institution: VFK - Verein fuer Krebsforschung - Society for Cancer Research - Arlesheim - Switzerland. Teachers: Stephan Baumgarter and Maria Olga Kokornazyck.
- Maimônides University - Buenos Aires - Argentina. Teacher: Francisco Eizayaga.
- Institution: Bion Institute - Lyubliania, Slovenia.
- Institution: DiagnOx Laboratory, Cherwell Innovation Center, Upper Heyford, Oxon, United Kingdom
- Institution: Hyland´s Homeopathy - USA

Professor: Selene Dall ’Acqua Coutinho
Partnership: IPÊ – Institute for Ecological Research
Projects involved: Malassezia spp. and dermatophytes in auditory flue and anteater fur (Myrmecophaga tridactyla) free living
Research of dermatophytes in soil of armadillo burrows of the Pantanal Mato-Grossense (Mato Grosso Swamplands)

Partnership: College of Nursing - Federal University of Alagoas
Project involved: Malassezia spp. in the cutaneous microbiome of preterm neonates in the Intensive Care Unit of a public hospital

Professor: Thiago Berti Kirsten
Partnership: Department of Pathology - FMVZ- USP
Project involved: Autism and dopaminergic system: analysis of sexual differences, molecular and behavioral aspects

b) Intercâmbios internacionais
Prof. Maria Anete welcomed student Alicia Herrera for traineeship, for the Master’s Degree in Veterinary Medicine from the Universidad Autónoma de Nuevo León in México, being guided by Prof. PhD. Uziel Castillo Velázquez. Her stay in Brazil enabled the international cooperation agreement between Paulista University and Universidad Autónoma de Nuevo León, that will be consolidated upon carrying out of the Ph.D. project with Alicia Herrera’s coming in 2020, involving the participation of Dr. Velázquez, as associate professor. In Mexico, professor Dr. Velázquez's group is the only one who works with microsporidia in Veterinary Medicine, especially in rabbit raising. In Brazil, our group is a reference in microsporidia, so this agreement will benefit the internationalization and strengthening of the 2 groups. There are already negotiations for the professors involved to move between universities to participate in newsstands, project idealization, and presentation of lectures. In this way, we hope that the realization of this project and the internationalization agreement will strengthen the 2 existing research groups in the Americas, excluding the already well-established groups in the United States, providing the idealization of a research network on microsporidiosis and broadening Brazil’s visibility. Thus, the Program demonstrates its ability to receive foreign students for Post-Graduate and training, thus enhancing their scientific maturity.

Professor: Paulo Ricardo Dell’Armelina Rocha
- Department of Veterinary Sciences, University of Turin, Italy
- Laboratory / Institution: Division of Pediatric Infectious Diseases, The Johns Hopkins University

Research aid obtained in progress

Project: Differentiation of B-1 Lymphocytes in Insulin-Producer Cells: Involved Mechanisms and Process Optimization.
Responsible Professor: Profa. Dra. Maria Anete Lallo
Development Agency: FAPESP (Lawsuit No 2017/06733-0)
Term: from 07/01/2017 to 06/31/2019
Amount: R$ 46,000.00 and USD $ 20,000.00

Project: Modulation of phagocytosis in vitro by homeopathic preparations in a co-culture model with Leishmania (L) amazonensis
Responsible Professor: Prof. Dr. Leoni Villano Bonamin
Organ of Foment: FAPESP
Process: 2014/00967-1
Duration: 2014 to 2016
Value: R$ 70.446,66 + US$ 29 312,66

Project: Possible prevention or treatment of the damage induced by exposure at the beginning of gestation to lipopolysaccharide in an experimental model of autism
Responsible Professor: Prof. Dr. Thiago Berti Kirsten
Organ of Foment: CAPES
Process: CAPES (AUXPE nº 1029/2014)
Duration: 23/04/2014 a 23/03/2019
Value: R$ 159.982,02

Project: Use of experimental model of depression in rats induced by lipopolysaccharide in the search for possible treatments
Professor Responsible: Professor PhD. Thiago Berti Kirsten
Fostering Body: CNPq-Universal (406835/2016-0)
Duration: from Aug/01/2016 to Aug/01/2019
Amount: R$ 24,800.00

Project: Influence of the administration of Buchinha-do-Norte on the serum concentration of corticosterone, TNF-a, IL-1, IL-6 and IL-10, testosterone, TGO, TGP and creatinine as indicators of behavioral alterations, on the reproductive system, liver and kidneys of Wistar rats
Professor Responsible: Professor PhD. Ivana Barbosa Suffredini
Fostering Body: Auxílio à Pesquisa Fapesp #2017/03470-9
Duration: from 04/2018 to 03/2019

Project: Assistance for article publication fee accepted in the magazine Plos Neglected Tropical Diseases titled: B-1 cell-mediated modulation of M1 macrophage profile ameliorates microbicidal functions and disrupt the evasion mechanisms of Encephalitozoon cuniculi
Responsible Professor: Prof. Ph.D. Maria Anete Lallo
Funding / Fostering Organization: Regular Programs / Research Aid / Scientific Publications / Article – Continuous Flow.  Process FAPESP: 2019/22308-3
Effective: Dec/01/2019 to May/31/2020
Amount: US$ 2,702.50

Project: Assistance for article publication fee accepted in the magazine Frontiers in Microbiology titled: Cyclophosphamide Treatment Mimics Sub-Lethal Infections With Encephalitozoon intestinalis in Immunocompromised Individuals.
Responsible Professor: Prof. Ph.D. Maria Anete Lallo
Funding / Fostering Organization: Program Assistance to Scientific Publications of the Paulista University
Effective: Dec/01/2019
Amount: US$ 2.950,50