Laboratory Extraction

Nucleus of Research in Biodiversity

Núcleo de Pesquisas em Biodiversidade

PRESENTATION

Objectives

A considerable number of drugs is originated from plants . Brazil is the country that has the richest biodiversity, comprises the Amazon Rainforest, Atlantic Forest, Cerrado and other important biomes, little unraveled by science. The Laboratory of Extraction of UNIP was designed to perform large-scale studies of these ecosystems. Thus samples of plant extracts are tested in biological, pharmacological or phytochemical models are then selected and split, so that the compound responsible for the activity is identified. Programs of this order are aimed at a large number of samples in a relatively short test time, using only a small amount of extract. The Extraction Lab is housed in the masters program in Veterinary Medicine and Dentistry, contributing to the increase of research in these areas.

Specific Objectives:

  • training of human resources in science ( MSc) in Veterinary Medicine , Dentistry , Pharmacy and other
  • develop studies to identify new drugs from Amazonian plants and Atlantic Forest
  • identify pharmacologically active substances of animal and human importance
  • promoting cooperation between educational institutions and research

History

In 1996, Dr.Drauzio Varella inaugurated, along with Dr. Riad Younes, the Laboratory of Extraction. Since that time, the botanical collections and technicians perform various plant species, belonging to a large number of families, in order to obtain organic and aqueous extracts from the ground plant material, according to techniques used in the National Cancer Institute / National Institutes of Health, United States. Over the years, other laboratories were assembled: the UNIP Herbarium and Botanical Laboratory, which receive samples of plants and where the taxonomic identification of each specimen is held under the lens of the curator of the herbarium. Today, the group conducts research in botany from different biomes of ecological importance, such as the Atlantic Forest, Amazon, Cerrado, Restinga, in order to study the dynamics of these forest areas, over time. Then opened the Antitumor Screening Laboratory, with funding from FAPESP, where cytotoxicity assays, with the goal of identifying extracts that inhibit the growth of human tumors in vitro are performed according to techniques used in the National Cancer Institute / National Institutes of Health, U.S. Later, it opened the Laboratory of Microbiology, whose biological models tested are intended to identify active plant extracts against bacteria of importance for human and veterinary importance. Along with the microbiology laboratory was set up a laboratory of Phytochemistry, where the fractionation and purification of compounds derived from plant extracts are performed. At the present stage, it is often necessary to use high-tech equipment, both for the isolation of chemicals to the elucidation of molecular structure. For this step is performed, an agreement was signed with the Institute of Education and Research of the Syrian-Lebanese Hospital, which has a laboratory prepared to receive spectrometers, chromatographs and nuclear magnetic resonance apparatus, gained from this partnership. Research groups in the IEP / HSL has been interested in performing biological in vitro with extracts of Extratoteca UNIP experiments. The spirit of research with extratoteca is that each extract to be tested in the largest possible number of biological assays. Thus , there will be an accumulation of pharmacological and phytochemical information related to each of them, adding value to Brazilian biodiversity.

Future Prospects

The potential of Brazilian biodiversity in the discovery of new drugs from plants is immense . The insertion of the extraction lab in Masters in Veterinary Medicine and Dentistry will enable the identification of drugs to be used as a tool for scientific research.

Area

Health Sciences

Area of ​​Knowledge

Pharmacy, Veterinary Medicine, Dentistry, Nursing, Physiotherapy, Biological Sciences, Chemistry

Graduate

The NPBio is part of the Graduate Programs in Environmental and Experimental Pathology and Dentistry and is equipped to conduct research in several areas of Health

Lines of Research

  • Screening of Brazilian plants with antitumor and antibacterial activity
  • Experimental models in immunopathology and immunotoxicology
  • Evaluation of the cytotoxic response of plant extracts
  • Evaluation of the antibacterial response of plant extracts
  • Evaluation of the antioxidant response of plant extracts
  • Evaluation of the toxicity of plant extracts
  • Essential oils in Amazonian plants. Seasonal variation and biological activity
  • Study of the activity of plant extracts against Escherichia coli
  • Efficacy of natural products used in nursing care auxiliary products
  • Evaluation of the influence of medicinal plants administered to pregnant rats on the behavior of pups challenged by estersse in the stage of young adults
  • Evaluation of the activity of plant extracts on dental biofilm

FACULTY / RESEARCHERS

Professor Dr. Antonio Drauzio Varella

Prof. Dr. Antonio Drauzio Varella

Lattes

Professor Dr. Riad Naim Younes

Prof. Dr. Riad Naim Younes

Lattes

Profª Drª Ivana Barbosa Suffredini

Profa. Dra. Ivana Barbosa Suffredini

Pharmaceuticals, has an MS and Ph.D in Pharmaceutical Sciences, Drug and Medicines area. She is a professor of Pharmacognosy and pharmacobotany the course of Pharmacy and professor of UNIP courses Postgraduate Veterinary Medicine and Dentistry. His emphasis of study is the identification of new drugs with antitumor and antibacterial activity of plant extracts from Amazonian plants and the Atlantic Forest. Heads the Laboratory of Extraction of UNIP since its foundation in 1996, which set up and has been managing its expansion process in order to provide access to students interested in developing scientific activities. Was responsible for transferring the technology required to implement the laboratory extraction, in which the plant extracts are obtained, and the antitumor screening laboratory, in which the extracts are tested against human tumor cells. The technology used is from the National Cancer Institute / National Institutes of Health, the American institution leader in cancer research and global reference center.
Lattes
Email: extractlab@unip.br
Telephones: (11) 3170-3776/3717 e FAX: (11) 3170-3978

Prof. Dr. Mateus Luis Barradas Paciencia

Prof. Dr. Mateus Luis Barradas Paciencia

He is Biologist, Master in Botany from the University of São Paulo (USP) and doctoral student at the Department of Botany of the USP. At UNIP he is curator of the Herbarium at Paulista University, and one of those responsible for studies on diversity of plant communities (trees and ferns) in the Rio Negro forest researchers, developed by the university. He was a founding member of the Dryads Research Institute and Biodiversity Conservation (NGO based in Ilheus, BA, which also participates) and researcher Project RestaUna (www.restauna.org.br), a project that investigated the effects of forest fragmentation on Bahian south over existing biological communities there during the years 1998-2002 Atlantic. Developed studies of fragmentation and communities of terrestrial ferns on community ecology of ferns of Atlantic Southeast (Ribeira Valley) and on development of native trees in the Serra do Mar, the latter performed during the period in which he worked on with Cetesb (1995-1997). Currently, besides Curator of the Herbarium UNIP is also a consultant in the area of ​​environment, conducting botanical surveys for environmental licensing.
Lattes

Prof. Wilson Roberto Malavasi

Prof. Wilson Roberto Malavasi

Responsible for the UNIP Environmental Schools (Sea School in Angra dos Reis - RJ; Nature School in Manaus- AM).
Coordinator Special Projects of UNIP.
Project Coordinator of Scientific Research Collections of Plants, Manaus - AM.
Principal of UNIP Manaus - AM.
Operations Director at MIX TV.

TECHNICIAN FACULTY

Sergio Alexandre Frana

Sergio Alexandre Frana

Pharmacist, graduated from Universidade Paulista - UNIP. In the project acts as responsible for biological assays in tumor cells. Part of the team that performs the collections of botanical material.

Luiz  Fernandes Coelho

Luiz Fernandes Coelho

Mr. Luiz , as he is known, is one of those people that we like right away at first glance. Intelligent, wise, good conversation, is a sort of icon for the research group of UNIP. Now retired, was an official of the INPA for almost fifty years, where he worked alongside some of the world's leading botanists knowledgeable and proficient in the Amazon flora, as Sir Prance Ghillean, Dr. William Roberts and Dr. Alwyn Gentry to. That way your plants Luiz knows like few others, despite not having graduated from college. It is a "practical botanist". Key part of the studies undertaken by the group.

>Osmar Barbosa Ferreira

Osmar Barbosa Ferreira

He is from Canutama interior of Amazonas. He has already been fisherman, hunter, rubber farmer. The need for self - sufficiency as a child made ​​Osmar develop some skills that only local people can, like to climb trees with amazing speed. Operates in the research group of UNIP as a collector of botanical and technical laboratory equipment, in Manaus.

Lucielda Ramos dos Santos

Lucielda Ramos dos Santos

She is from Panelas, PE, came to Sao Paulo 15 years. Today, she supports for projects taking care of cleaning the lab and materials, made under strict control Shows huge interest in techniques involved in cleaning both in the work of colleagues.

LABORATORIES

Herbarium UNIP

Laboratory Extraction
he idea of researching the Amazon's biodiversity took place in the early '90s, when Dr.Drauzio Varella traveled to the Amazon and found that, in dazzlingly green landscape tree leaves had completely taken by fungi, while others remained tender and intact. It realized there was a huge scientific potential in the middle of the Amazon and a vast gap in large-scale research with the goal of identifying scientific instruments that aided in studies on the therapy of cancer, infectious and other diseases. In the late 1990s, under the concepts employed in similar laboratories of the National Cancer Institute, linked to the National Institutes of Health, USA, Laboratory of Extraction of UNIP was assembled with the goal of obtaining the highest number of plant extracts Brazilian plants, in a systematic way, so that they were subjected to biological and pharmacological tests. Today, the laboratory has a extratoteca, or a library of plant extracts, consisting of over 2,000 plant extracts from the Amazon and Atlantic Forest, which remain stored at - 20° C, in cold chambers. Each sample of plant is subjected to extraction process, two of which are extracts obtained an organic and an aqueous, so that a significant portion of the compounds present in the plant is withdrawn or apolar extract, or polar extract. The extracts are, in turn, diluted in suitable solvents and tested in biological models. The active extracts are fractionated and the compound responsible for the activity is isolated, identified and tested in the biological model, so that its biological activity is confirmed and compared with reference substances. A major goal of obtaining and maintaining extratoteca is to generate a constant source of samples to be tested in various biological models, preferably developed in vitro, and thus optimize the identification of new pharmacological tools to be employed in studies of various diseases.

Laboratory of Microbiology
The Microbiology Laboratory was set up with the objective of evaluating the antimicrobial response of plant extracts against microorganisms of importance in human and animal pathologies. Staphylococcus aureus and Enterococcus faecalis and two Gram - negative Pseudomonas aeruginosa and Escherichia coli - over 1200 extracts against four bacteria, two Gram positive were tested. With the introduction of Extraction Laboratory courses in Masters in Dentistry and Veterinary Medicine, there was an expansion in the number of antimicrobial trials and statements started to be tested in other bacteria of specific interest to these areas, such as Streptococcus mutans, Streptococcus sanguis, Candida albicans, gingivalis and Prevotella intermedia, important in periodontal disease and decays. Porphyromonas. The models employed in the assays are validated in order to conduct the analysis of a large number of samples per week, using a minimum amount of sample.

Laboratory Screening Antitumor
his laboratory was established with the objective of evaluating the cytotoxic activity against human malignant tumors grown in vitro , extracts and other samples. The use of our equipment and lab supplies are funded by FAPESP. This laboratory was established within the parameters established at the National Cancer Institute, linked to the National Institutes of Health, United States , where there was technology transfer. Currently, the panel contains 6 cells Laboratory tumor cell lines: breast, prostate, lung, colon, central nervous system, and leukemia. 6 against these cells have been tested over 1200 extracts for cytotoxic activity of these and one hundred twenty is presented lethal to 15% or more of the cells cultured in growth compared to untreated controls. These extracts will be fractionated and tested again in the biological model, and will be evaluated for chemical composition of the classes present. New cells will be introduced in our panel , referring to malignant tumors of the head and neck, and eventually some of veterinary importance.

EVENTS

  • XX Symposium on Medicinal Plants of Brazil. São Paulo, 16 September 19, 2008, the Convention Center Frei Mug
  • VIII Scientific Meeting and the Tenth Meeting of the Scientific Initiation, Sao Paulo, 3-5 September UNIP , Indianapolis Campus.

THESIS

Not yet published.

PUBLICATIONS

COVENANTS

The UNIP has established an agreement with the Union , specifically with IBAMA - Brazilian Institute of Environment and Renewable Natural Resources in order that the project bioprospecting of novel anticancer drugs and antibiotics could be realized. The Management Board of Genetic Heritage is the institution responsible for providing access to genetic resources to UNIP, and IBAMA, as managing agent of conservation in which UNIP works , is the body responsible for providing the license for collecting the plant material with which the plant extracts are obtained and subsequently tested in vitro biological models.

Recently, an agreement with the Institute of Education and Research of the Syrian -Lebanese Hospital, so the step to elucidate the chemical components of plant extracts are separated and identified was established . Techniques developed chromatography and spectrometry are used to determine the chemical structure of natural products and chromatographic profiles of plant extracts. In addition , research groups established in the IEP / HSL also cooperate with the research group of the Laboratory of extraction so that the plant extracts are tested in other biological models.

RECOMMENDED

CONTACT

Telephones: (11) 3170-3776/3717
FAX: (11) 3170-3978
Email: extractlab@unip.br